I-CeuI from New England Biolabs

Product Specs
ItemI-CeuI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0699L
Quantity2500 units
Concentration5,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

TAACTATAACGGTCCTAA/GGTAGCGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.,New England Biolabs,Cat#R0629S PI-SceI,New England Biolabs,Cat#R0696S I-CeuI,New England Biolabs,Cat#R0699S PacI,New England Biolabs,Cat#R0547L ApaI,New England Biolabs,Cat#R0114S MfeI-HF,New England Biolabs,Cat#R3589L XhoI,New England Biolabs

(2) Strand asymmetry influences mismatch resolution during a single-strand annealingGenome BiologyDecember 1, 2022Victoria O. Pokusaeva, ArÃ¡nzazu Rosado Diez, Lorena Espinar, Albert TorellÃ³ PÃ©rez, Guillaume J. Filionthis, non-integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 min heat inactivation at 65 °C. All enzymatic reactions were

(3) Systematic analysis of intrinsic enhancer-promoter compatibility in the mouse genomeMolecular CellJuly 7, 2022Miguel Martinez-Ara, Federico Comoglio, Joris van Arensbergen, Bas van Steenselbrief, the combination of barcode and element(s) was excised from the plasmid by digestion with I-CeuI(#R0699S, NEB); this fragment was circularised; remaining linear fragments were destroyed; and circular fragments

(4) MegaGate: A toxin-less gateway molecular cloning toolSTAR ProtocolsDecember 17, 2021Christian Kramme, Alexandru M. Plesa, Helen H. Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E. Kohman, Pranam Chatterjee, George M. Church11789020 I-SceI (5U/ ul),New England Biolabs,Cat: R0694 I-CeuI (5U/ ul),New England Biolabs,Cat: R0699 10× CutSmart Buffer,New England Biolabs,Cat: B7204 T5 Exonuclease,New England Biolabs,Cat: M0663 Gibson Assembly Master Mix

(5) An integrated pipeline for mammalian genetic screeningCell Reports MethodsOctober 25, 2021Christian Kramme, Alexandru M. Plesa, Helen H. Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E. Kohman, Pranam Chatterjee, George M. Church11,791,020)•1 μL (5U) of the MegaNuclease I-SceI (NEB R0694)•1 μL (5U) of the MegaNuclease I-CeuI (NEB #R0699)•5 μL of 10X CutSmart Buffer (NEB B7204).•Nuclease free water was then utilized to bring the reaction

(6) A standard for near-scarless plasmid construction using reusable DNA partsNature CommunicationsDecember 1, 2019Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang ZhouRE-digestion solution used in Supplementary Fig. 11 contained 1 µL of each RE (NotI [R3189S, NEB] and I-CeuI [R0699S, NEB]), a certain quantity of plasmids (~1 µg), 5 µL of 10X CutSmart® Buffer (B7204S, NEB), and ultrapure

(7) Fortilin binds IRE1α and prevents ER stress from signaling apoptotic cell deathNature communicationsMay 26, 2017Pinkaew D, Chattopadhyay A, King MD, Chunhacha P, Liu Z, Stevenson HL, Chen Y, Sinthujaroen P, McDougal OM, Fujise K.sequencing to verify the integrity of the construct, the targeting vector was linealized by I-CeuI (R0699S, New England BioLabs, Ipswich, MA, USA) and electroporated into C57BL6 embryonic stem cells (ESCs).

(8) Genome-wide mapping of autonomous promoter activity in human cellsNature BiotechnologyFebruary 1, 2017Joris van Arensbergen, Vincent D. FitzPatrick, Marcel de Haas, Ludo Pagie, Jasper Sluimer, Harmen J. Bussemaker, Bas van Steenselassociate the barcodes with the linked genomic fragments, we digested 4 μg SuRE library with I-CeuI (#R0699S; NEB), followed by magnetic bead purification (1:1 ratio beads:DNA solution). Of this, 2 μg was self-ligated

(9) An efficient method of directly cloning chimpanzee adenovirus as a vaccine vectorNature ProtocolsNovember 1, 2010Dongming Zhou, Xiangyang Zhou, Ang Bian, Hua Li, Heng Chen, Juliana C Small, Yan Li, Wynetta Giles-Davis, Zhiquan Xiang, Hildegund C J Ertlcat. no. V0269S) SwaI (New England Biolabs, cat. no. R0604S) I-CeuI (New England Biolabs, cat. no. R0699S) PI-SceI (New England Biolabs, cat. no. R0696S) SgfI (Promega, cat. no. R5104) Eco47III (Promega,

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I-CeuI

I-CeuI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (9)

Reviews

I-CeuI from New England Biolabs

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